4th International Conference and Exhibition on Pharmacovigilance & Clinical Trials
Title: Mechanisms of antibiotic resistance of gram negative strains isolated from nosocomial infections. The active compounds derived from plant extracts by chromatographic methods involved in blocking resistance mechanisms of gram-negative strains
Biography: Hosu Anamaria Delia
We could say that life is unthinkable without antibiotics. However, immediately after the appearance of a new antibiotic, at the same time it appeared as new form of antimicrobial resistance. The prevalence of multidrug-resistant strains isolated from nosocomial and community infections increased lately. The traditional treatments of infectious diseases based on the microbicidal active compounds led, due to the overuse, the selection and the emergence of phenomen of resistance and MDR. Pathogenic strains therapeutic exposing to high concentrations of antibiotic creates sever pressure conditions and induces high-level resistance. Recycling antibiotics is a short-term measure, because resistant strains do not disappear, and when reintroduced into the antibiotic therapy resistance genes will be selected soon. In those situations where antibiotics seem to be compromised, it is necessary to find substitutes whose action are to be lasting and without any secundare. Medicinal plants seem to be an inexhaustible source of active compounds whose antibacterial activity is known ancient times. The number and mode of action of compounds obtained from extracts of medicinal plants are not fully elucidated. This study is intended to assist those immunocompromised persons with multiple infections, which the body does not respond to standard treatment with antibiotics. In vitro studies have been made on isolated nosocomial infections with multiple resistance to antibiotics. To highlight the mechanisms of resistance of strains, we used PCR. The active compounds of plant extracts were determined by GC and HPLC and antibacterial activity demonstrated by diffusion method.