2015 PhD at the Faculty of Biology, University of BucharestrnPresentation2014 :rn1.Fungal infectionprevalencein humans and animals, methodsof diagnosis and treatmentofhydro-alcoholextractofplants. International Symposium of Academy sciences of Moldova.rn2.Methods for restorimg wooden monuments altered under the action of fungus. rn3.Treatment of wood objects using compouds from plant extracts with antifungal effect.International Symposium of Drobeta.rnPublications of Nature Sciencesjournal Drobeta,2015:rn1.Bioresonance- a method for infectious furnace volume location.rn2. The enzymatic activity of fungi. The mycoparasitic effect of the Trichoderma strains has ecological implications since. rn3. Natural colors painter wood.rnrn
Despite the development of the pharmaceutical industry, currently at least 30 000 people die every year in Europe due to infections caused by antibiotic-resistant microorganisms: Entercoccus spp. vancomycin-resistant (VRE), Enterobacteriaceae (Escherichia coli, Klebsiella pneumoniae) resistant to cephalosporins, third generation Enterobacteriaceae (K. pneumoniae) carbapenems-resistant and non-enteric (Pseudomonas aeruginosa) resistant to carbapenems. For now almost every existing antibiotic, bacteria have developed a resistance factor that protects them from the action. For each resistance factor, pharmaceutical companies have developed a stronger antibiotic - until today. In the battle between bacteria and antibiotics begin to tilt the balance of victory by these microorganisms. In these circumstances, return to traditional medicine seems to be the solution. In order to determine the antibacterial activity of the compounds of Syzygium aromaticum buds extract against bacteria isolated from nosocomial infections MDR, we used the disc-diffusion method and inoculation of 96-well plate BHI. Screening of genes coding β- lactam antibiotics resistance was performed by PCR. The active compounds of the ethanol extracts and essential oil of S. aromaticum were determined by HPTLC, respectively by GC. Gram positive and Gram negative strains isolated from nosocomial infections showed genotypic resistance characteristics lactam antibiotics. Hydroalcoholic extracts and essential oil of S. aromaticum were active against all MDR bacteria. Eugenol is the main component of the extract of E. caryophyllata. In conclusion, the extracts of S. aromaticum can successfully replace antibiotics whose action against MDR bacteria proved to be ineffective.
Javeria Fazal will complete her Master’s Program in coming July (2015) at the age of 28 years from University of Calabria,Italy.She has successfully completed Pharm D (Doctor of Pharmacy) from Pakistan.After it she was entitled as a Quality Control Assistant leading to Quality Control Manager in Pharmaceutical firm and also performed duties of Hospital Pharmacist in hospital.In 2013 she came to Italy with great ambition of Clinical findings so this is her first research throughout her academic career and she wants to proceed it to her PhD Program. rnrn
The aim of the study was to investigate if the average Pakistanis and average Italian kidney post transplanted patients have same episodes of adverse events after intake of cyclosporine, tacrolimus and corticosteroids and also to green light the safest as well as hazardous drug among these three and to explain the appropriate reasons for effectiveness and ineffectiveness of the respective drug. The subjects were randomly selected including 706 Pakistani and 568 Italian patients both male and female with inclusion criteria of teen age and geriatric patients as well to estimate the clinical response. Blood sample was taken from each subject in order to determine the amount of creatinine, glucose, hemoglobin and cholesterol which are ultimate parameters for cardiovascular and cerebrovascular accidents..Urine sample was also taken in order to determine level of protein. Also DFG (Delayed Graft Function) was observed in these subjects. Thirty one percent of Italian men and 26% of Italian women (out of 568 Italian patients) suffered from hypercreatininemia and hyperproteinuria including hemoglobinemia and hypercholesterolemia and only a minority of sample population was subjected to hyperglycemia whereas 22% of Pakistani men experienced from same adverse effect but for Pakistani women the percentage was 21.50 (out of 706 Pakistani patients). The sample was selected from different age groups but it was observed that majority of adverse effects were found between age group 46-55 and the percentage of occurrence of adverse effects was found to be least between age group 17-25. It was observed that for immuno-compromised patients DGF, mortality rate and risk of transplant failure has been shown minimum for corticosteroids, intermediate for tacrolimus and maximum for cyclosporine and for immuno-competent patients, tacrolimus was effective drug. Abnormal kidney function gives favourable place for growth of many bacteria and ultimately level of protein become raise and there will start competitive binding between these proteins and cyclosporine as cyclosporine itself is composed of 11-amino acids so those having same ‘R’ functionality with the attaching side of cyclosporine will compete and finally cyclosporine will not be available to bind with its receptor and will give rise to series of adverse events. Also it was observed that patient’s adherence was maximum with corticosteroids as they produce synergistic effect with adrenal glands steroid production thereby suppressing immune system with diminished adverse effects and was gradually decreasing as we move from tacrolimus to cyclosporine. These data suggest that immuno-suppressant drugs should be monitored with special care depending upon the immune status of the patient.